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KMID : 0923620110110040196
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Immune Network
2011 Volume.11 No. 4 p.196 ~ p.202
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Activin A Stimulates Mouse APCs to Express BAFF via ALK4-Smad3 Pathway
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Kim Jae-Hee
Seo Goo-Young
Kim Pyeung-Hyeun
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Abstract
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Background:B cell-activating factor belonging to the TNF family (BAFF) is primarily expressed by macrophages and dendritic cells, and stimulates B cell proliferation, differentiation, survival, and Ig production. In the present study, we explored the effect of activin A on BAFF expression by APCs.
Methods: To investigate the effect of activin A on BAFF expression by mouse APCs, we measured the level of BAFF expression at the transcriptional and protein levels using RT-PCR and ELISA.
Results: Activin A markedly enhanced BAFF expression in mouse macrophages and dendritic cells at both the transcriptional and protein levels. SB431542, an activin receptor-like kinase 4 (ALK4) inhibitor, completely abrogated activin A-induced BAFF transcription. Furthermore, overexpression of DN-Smad3 abolished activin-induced BAFF expression at the transcriptional and protein levels.
Conclusion: These results demonstrate that activin A can enhance BAFF expression through ALK4-Smad3 pathway.
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KEYWORD
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Activin A, BAFF, APCs, ALK4, Smad3, Ig class switch recombination
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